DNA

Part:BBa_K2878000:Design

Designed by: From literature   Group: iGEM18_SSHS-Shenzhen   (2018-09-28)


T7 promoter sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We just add the T7 promoter oligo sequence to the 5' end of our shRNA template sequence.


Source

T7 promoter is from the genome of phage T7,the T7 promoter sequence is used extensively in molecular biology due to its extremely high affinity for T7 RNA polymerase and thus high level of expression.

References