DNA
Part:BBa_K2878000:Design
Designed by: From literature Group: iGEM18_SSHS-Shenzhen (2018-09-28)
T7 promoter sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We just add the T7 promoter oligo sequence to the 5' end of our shRNA template sequence.
Source
T7 promoter is from the genome of phage T7,the T7 promoter sequence is used extensively in molecular biology due to its extremely high affinity for T7 RNA polymerase and thus high level of expression.